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qPCR is fairly easy to accomplish as compared to flow cytometry that requires significant abilities for reproducible benefits. In actual fact, move cytometric enumeration of microbial cells was initially restricted to pure cultures [31] and continue to stays complicated when carried out in intricate matrices [32].
qPCR-dependent quantitative microbiome profiling is relevant also for samples that contains a considerable volume of host or non-bacterial DNA, by which bacterial density can not be reliably estimated by complete DNA produce [five].
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In contrast to circulation cytometry that counts cells, qPCR and NGS both concentrate on bacterial DNA, together with extracellular DNA derived from lysed germs. Extracellular DNA may be intrinsic or end result within the differential lysis of Gram-beneficial and damaging microbes during the widespread freeze-thawing before fecal DNA extraction.
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